DNA

Part:BBa_K5412002:Design

Designed by: Divakar Kalivarathan   Group: iGEM24_SVCE-CHENNAI   (2024-10-02)


CFTR mutant


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 486
    Illegal EcoRI site found at 2903
    Illegal SpeI site found at 2681
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 486
    Illegal EcoRI site found at 2903
    Illegal SpeI site found at 2681
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 486
    Illegal EcoRI site found at 2903
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 486
    Illegal EcoRI site found at 2903
    Illegal SpeI site found at 2681
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 486
    Illegal EcoRI site found at 2903
    Illegal SpeI site found at 2681
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2705


Design Notes

Critical design considerations for the CFTR mutant sequence include ensuring accurate mutation incorporation, maintaining reading frame integrity, and avoiding off-target effects. It's essential to confirm the mutation does not introduce unintended errors. Codon optimization and ensuring proper folding of the CFTR protein are crucial for studying disease mechanisms and potential therapies.



Source

Synthetic generate.

References